Microbial diversity was investigated in the soil samples from Himachal Pradesh and Kumaun Himalaya. Phylogenetic analysis of all bacterial strains confirms the presence of Proteobacteria (55%), Firmicutes (27%), Actinobacteria (18%) and Bacteroidetes (6%) like mesophilic microbial communities in the study area. Shannon Index was used as a tool for bacterial diversity assessment. Isolated bacterial strains were further screened for extracellular crude carbonic anhydrase (CA) production. Pseudomonas spp. is able to produce extracellular CA, while other bacterial strains showed intracellular activity. 16s rRNA sequencing and FT-IR was used to identify the isolates and CA application in CO₂ mineralization.