In this work, laboratory experiments and numerical simulations were used to investigate the transport of solute through stratified porous media. Experiments were performed using nonreactive solute transport through an experimental model of stratified porous media of different lengths. The aim of conducting the experiments was to evaluate the effect of the length of mobile-immobile domain on estimation of first-order mass transfer coefficient. The velocity was also changed for different lengths of stratification for understanding the behavior of mass transfer coefficient. The estimated values of mass transfer coefficient were analyzed for interdependency with length, velocity, and dispersion coefficient. It was found that mass transfer coefficient has an inverse relation with dispersion coefficient and also varies with the length of the mobile-immobile region and velocity. Finally, the variable behavior of mass transfer coefficient is obtained when the transport media are under the influence of physical nonequilibrium.